| CBDPSS, catalog number 014SS, seems insoluble in water. |
| Hydrolysis products of sulfo-NHS esters lower pH of the solution, which leads to their poor solubility in water. To obtain clear stock water solution make sure resulting pH is at least neutral. For example, you can reconstitute the product in 0.1 or 0.2 M Na2HPO4, but use it immediately, as hydrolysis will occur at neutral-to-basic pH.
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| Can I use DXMSMS Match software for non-cleavable and non-labeled crosslinkers? |
| Yes, you can use the program for non-labeled or non-cleavable crosslinkers. In these cases doublet or cleavage product scores will be missing.
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| Can I purchase light and heavy isotopic forms of the reagents non-mixed? |
| Yes, you can purchase light and heavy isotopic forms of the reagents separately. Pay the order as usual via PayPal and notify us with email that you need light and heavy forms separately.
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| How to pay the order with credit card via your website? |
| Go to our home page and click "Add to Cart" button against each product you want to purchase. It will transfer you to PayPal webpage, where you can adjust items and number for each item, proceed to checkout an pay with your credit card as PayPal guest.
The system will generate automatic email of the notification of the payment with your shipping address, which will be sent to us.
To pay with credit card already received orders use "Pay Now" button on the bottom of the home page and enter order number in descriptions box.
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| What is advantage of isotopically-coded crosslinkers? |
| When used as a mixture of light and heavy isotopic forms resulting crosslinks manifest as
specific doublets of peaks in mass spectra. This allows univocal detection of the crosslinks. |
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| What is advantage of cleavable crosslinkers? |
| Combinatorial nature of the inter-peptide crosslinks hampers
theirs analysis and identification. Cleavage allows to analyze individual
peptides constituting crosslinks. |
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| What is advantage of isotopically-coded cleavable crosslinkers? |
| As both uncleaved and cleaved crosslinked peptides are
isotopically coded, combination of the isotopic coding and cleavage
allows to establish a specific mass relation between uncleaved crosslink and
produced by cleavage individual peptides constituting the crosslink, thus
enabling confident identification of the crosslinks. In addition dead-end
and intra-peptide crosslinks can be readily identified based on specific
mass losses due to cleavage of the crosslinker. |
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