| What is advantage of isotopically-coded crosslinkers? |
| When used as a mixture of light and heavy isotopic forms resulting crosslinks manifest as
specific doublets of peaks in mass spectra. This allows univocal detection of the crosslinks. |
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| What is advantage of cleavable crosslinkers? |
| Combinatorial nature of the inter-peptide crosslinks hampers
theirs analysis and identification. Cleavage allows to analyze individual
peptides constituting crosslinks. |
|
| What is advantage of isotopically-coded cleavable crosslinkers? |
| As both uncleaved and cleaved crosslinked peptides are
isotopically coded, combination of the isotopic coding and cleavage
allows to establish a specific mass relation between uncleaved crosslink and
produced by cleavage individual peptides constituting the crosslink, thus
enabling confident identification of the crosslinks. In addition dead-end
and intra-peptide crosslinks can be readily identified based on specific
mass losses due to cleavage of the crosslinker. |
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